Journal: BioMed Research International

Article Title: Nav1.7 via Promotion of ERK in the Trigeminal Ganglion Plays an Important Role in the Induction of Pulpitis Inflammatory Pain

doi: 10.1155/2019/6973932

Figure Lengend Snippet: Expression of Nav1.7 in the TG pulpal afferent neurons. (a) Immunohistochemical staining with Nav1.7. (b) Fluorogold-labeled primary afferents in the TG section. (c) Merged image of (a) and (b). The arrow indicates a Nav1.7 positive pulpal afferent neuron. Scale bar: 100 μ m.

Article Snippet: The sections were then incubated for 48 h at 4°C with rabbit anti-FG (1:1000, Sigma) and mouse anti-Nav1.7 (1:1000, Sigma) [ – ].

Techniques: Expressing, Immunohistochemical staining, Staining, Labeling

Journal: BioMed Research International

Article Title: Nav1.7 via Promotion of ERK in the Trigeminal Ganglion Plays an Important Role in the Induction of Pulpitis Inflammatory Pain

doi: 10.1155/2019/6973932

Figure Lengend Snippet: Expression of Nav1.7 in the TG pulpal afferent neurons. (a–d) Immunostaining showing Nav1.7 expression in the TG. The protein expression of Nav1.7 increased in the TG in a rat model of pulpitis from days 3 through 14. The highest level of protein expression was observed on day 14. Each group included four rats. Scale bar: 100 μ m.

Article Snippet: The sections were then incubated for 48 h at 4°C with rabbit anti-FG (1:1000, Sigma) and mouse anti-Nav1.7 (1:1000, Sigma) [ – ].

Techniques: Expressing, Immunostaining

Journal: BioMed Research International

Article Title: Nav1.7 via Promotion of ERK in the Trigeminal Ganglion Plays an Important Role in the Induction of Pulpitis Inflammatory Pain

doi: 10.1155/2019/6973932

Figure Lengend Snippet: Western blot analysis of Nav1.7 expression in the TG of control and pulp-exposed rats. The protein expression of Nav1.7 increased in the TG from days 3 through 14 in a rat model of pulpitis. The highest level of protein expression was found on day 14. ∗ indicates a statistically significant difference (p <0.05) between the two groups. Each group included four rats.

Article Snippet: The sections were then incubated for 48 h at 4°C with rabbit anti-FG (1:1000, Sigma) and mouse anti-Nav1.7 (1:1000, Sigma) [ – ].

Techniques: Western Blot, Expressing

Journal: BioMed Research International

Article Title: Nav1.7 via Promotion of ERK in the Trigeminal Ganglion Plays an Important Role in the Induction of Pulpitis Inflammatory Pain

doi: 10.1155/2019/6973932

Figure Lengend Snippet: Effects of pulp exposure on mechanical hypersensitivity. The Nav1.7 in TG from the sham, pulpitis rats, and rats with pulpitis given PF04856264 (an Nav1.7 antagonist, 30 mg/kg) on postoperative day 14. PF04856264 significantly increased the facial mechanical pain threshold in the pulpitis rats (n=8, ∗∗ p<0.01). When PF04856264 was administered, the increase in threshold started at 0 h after and reached a maximum at 1h after injection.

Article Snippet: The sections were then incubated for 48 h at 4°C with rabbit anti-FG (1:1000, Sigma) and mouse anti-Nav1.7 (1:1000, Sigma) [ – ].

Techniques: Injection

Journal: BioMed Research International

Article Title: Nav1.7 via Promotion of ERK in the Trigeminal Ganglion Plays an Important Role in the Induction of Pulpitis Inflammatory Pain

doi: 10.1155/2019/6973932

Figure Lengend Snippet: Western blotting analysis of ERK expression in the TG of control and pulp-exposed rats. The Nav1.7 in TG from the sham, pulpitis rats, and rats with pulpitis given PF04856264 (an Nav1.7 antagonist, 30 mg/kg) in the same blot on postoperative day 14. Representative gel images and summary data show that the intensity of ERK is increased in pulpitis rats compared with that in the sham rats.

Article Snippet: The sections were then incubated for 48 h at 4°C with rabbit anti-FG (1:1000, Sigma) and mouse anti-Nav1.7 (1:1000, Sigma) [ – ].

Techniques: Western Blot, Expressing

Journal: BioMed Research International

Article Title: Nav1.7 via Promotion of ERK in the Trigeminal Ganglion Plays an Important Role in the Induction of Pulpitis Inflammatory Pain

doi: 10.1155/2019/6973932

Figure Lengend Snippet: Western blot analysis of Nav1.7 expression in the TG of control and pulp-exposed rats. The ERK in TG from the sham, pulpitis rats, and those given PD98059 (an ERK antagonist, 10.0 μ M/L) in the same blot on postoperative day 14. Representative gel images and summary data show that representative gel images and summary data show no significant difference in Nav1.7 protein levels in pulpitis rats compared with the sham rats.

Article Snippet: The sections were then incubated for 48 h at 4°C with rabbit anti-FG (1:1000, Sigma) and mouse anti-Nav1.7 (1:1000, Sigma) [ – ].

Techniques: Western Blot, Expressing